Method for efficient and rapid propagation of emmenopterys henryi

A method for efficient and rapid propagation of Emmenopterys henryi, comprising the following steps:

• culturing a tender stem with buds from an Emmenopterys henryi seedling as an explant on an initiation medium to obtain a sterile seedling;
• taking leaves of the sterile seedling and inducing differentiation of the leaves on a differentiation medium to obtain adventitious buds;
• subjecting the adventitious buds to proliferation culture on a proliferation medium to obtain proliferated adventitious buds;
• culturing the proliferated adventitious buds on an elongation medium to obtain elongated adventitious buds;
• subjecting the elongated adventitious buds to rooting culture on a rooting medium to obtain tissue culture plantlets;
• and
• transplanting the tissue culture plantlets into a mixed substrate for hardening,
• wherein the differentiation medium is Murashige and Skoog (MS) medium containing 1.0 mg/L 6-benzylaminopurine (6-BA), 0.1 mg/L 1-naphthaleneacetic acid (NAA), and 0.01 mg/L thidiazuron (TDZ);
• and
• the elongation medium is MS medium containing 3.0 mg/L 6-BA, 0.1 mg/L indole-3-butyric acid (IBA), and 0.1 mg/L gibberellic acid (GA3).