Method for Light-Field Microscopy and Light-Field Microscope

Light-field microscope, comprising:

• a light source for providing illumination light,
• an illumination beam path for guiding the illumination light into a sample region,
• a two-dimensionally spatially resolving detector for detecting detection light coming from a sample in the sample region,
• a detection beam path with a microscope objective and a multi-lens array for guiding the detection light to the detector,
• a displacement device for adjusting a relative position between the sample and the microscope objective in the direction of an optical axis of the microscope objective and/or in the direction of at least one lateral direction perpendicular thereto and
• a control unit for controlling the displacement device and for evaluating measurement data from the detector, wherein the control unit is configured
• A) to control the displacement device in such a way that image data sets of the sample are successively acquired for in each case different relative positions between the microscope objective and the sample,
• B) to reconstruct a respective three-dimensional image stack of the sample from the individual image data sets (T01, . . . , T37),
• C) to control the displacement device on the basis of a dependence of a magnification of the light-field microscope on an axial coordinate which runs parallel to the optical axis, in such a way that adjacent image stacks in the various relative positions in step A) overlap in part, and
• D) to combine adjacent image stacks of the sample to form a three-dimensional overall image of the sample on the basis of a dependence of a magnification of the light-field microscope on the axial coordinate.