Compositions And Methods For Detecting Proteins In Protein Corona

A method for detecting one or more biomarker or a pattern of one or more biomarker associated with a disease, the method comprising:

• adding one or more small molecule to at least two biological samples each biological sample from different subjects diagnosed with the disease;
• adding one or more protein-binding agent having a surface capable of binding proteins to the biological samples and allowing a protein corona to form on the surface of the one or more protein-binding agent to produce a complex comprising the protein corona and the one or more protein-binding agent;
• and
• detecting the one or more biomarker or the pattern of one or more biomarker in the protein corona by an antibody-based technique or a proteomics technique.

A method of diagnosing a disease in a subject, the method comprising:

• adding one or more small molecule to a biological sample from the subject;
• adding one or more protein-binding agent having a surface capable of binding proteins to the biological sample and allowing a protein corona to form on the surface of the one or more protein-binding agent to produce a complex comprising the protein corona and the one or more protein-binding agent;
• and
• detecting one or more biomarker or a pattern of one or more biomarker associated with the disease in the protein corona by an antibody-based technique or a proteomics technique.