Systems and methods for real-time detection and capture of invasive cell subpopulations from co-cultures

Abstract

Systems and methods are disclosed for identifying invasive cells. In certain embodiments, the system is comprised of a first chamber comprised of media, and the first plurality of cells are healthy cells, diseased cells, or a mixture of both, a second chamber comprised of a filtration membrane and a third chamber comprised of media, and a second plurality of cells. The second plurality of cells produces one or more chemoattractants or chemorepellents, and the first, second, and third chambers are clipped together to form a chamber array. The chamber array is mounted to an electrical cell impedance sensing reader, and impedance readouts are collected from the electrical cell impedance sensing reader at predetermined time intervals. Then, invasive cells are extracted from under the filtration membrane of the second chamber, wherein the extracted invasive cells are characterized using the collected impedance readouts normalized over a predetermined time period.

Dependencies are inferred from the claim text.