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US 2026/0265822 A1Published application
Method for detecting polynucleotide using risc
AssigneesimfliBIO Inc.
InventorsSungchul HOHNG, Soochul SHIN
Life of this patent
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Abstract
Disclosed herein is a method for detecting small RNAs and discriminating single nucleotide polymorphism at a single molecule level, and specifically to a method for detection of small RNAs, whereby the detection and quantitation of small RNAs and the identification of single nucleotide polymorphism (SNP) can be achieved.
Claims13
A composition for detecting a polynucleotide, comprising a protein-nucleic acid complex that includes an Argonaute protein associated with a nucleic acid molecule,
- wherein the nucleic acid molecule comprises a nucleic acid sequence complementary to a target nucleic acid region in the polynucleotide and is labeled with a fluorophore, and
- wherein the polynucleotide to be detected is immobilized onto a surface of a detection chip,
- wherein the detection is achieved by measuring a fluorescence signal generated by the fluorophore when the nucleic acid sequence binds to the target nucleic acid region, at the temperature of 37° C. or less.
Dependencies are inferred from the claim text.