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US 2026/0265773 A1Published application
Transposition of nucleic acid constructs into eukaryotic genomes with a transposase from amyelois
AssigneeDna Twopointo Inc.
InventorsJeremy Minshull, Sridhar Govindarajan, Maggie Lee
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Abstract
The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
Claims27
1-20. (canceled)
A kit comprising:
- (a) a transposon comprising a heterologous polynucleotide between left and right transposon ends, wherein the left end comprises SEQ ID NO:
- 9 and the right end comprises SEQ ID NO:
- 10, wherein the transposon is transposable by a transposase having the sequence of SEQ ID NO:18;
- or
- (b) a transposase polypeptide, the amino acid sequence of which is at least 90% identical to the amino acid sequence of SEQ ID NO:
- 18, or a polynucleotide comprising an open reading frame encoding the transposase polypeptide, operably linked to a first heterologous promoter.
3 more elements
Dependencies are inferred from the claim text.