Methods of increasing the regeneration efficiency in plants
AssigneeInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences
InventorsChuanyou Li, Wentao Yang, Fangming Wu, Huawei Zhai, Chuanlong Sun, Lei Deng
Life of this patent
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Abstract
The invention relates to methods of increasing regenerative capacity in plants. In particular, the methods of the invention relate to increasing the levels and/or activity of peptides involved in local wound signalling and plant regeneration. Also described are plants expressing these peptides and well as methods of producing such plants.
Claims30
A tissue regeneration factor comprising an amino acid sequence as defined in SEQ ID NO:
- 2 or a functional variant or homologue thereof, wherein the homologue is selected from SEQ ID NO:
- 4, 6, 8, 10, 12, 14, 16, 18, 89 or 20 or a functional variant or homologue thereof of any of SEQ ID NOs 2, 4, 6, 8, 10, 12, 14, 16, 18, 89 or 20.
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A nucleic acid construct comprising a nucleic acid sequence encoding at least one of a tissue regeneration factor, a tissue regeneration factor precursor and a tissue regeneration factor receptor;
- wherein the tissue regeneration factor is selected from SEQ ID NO:
- 2 or functional variant or homologue thereof, wherein the homologue is selected from SEQ ID NO:
- 4, 6, 8, 10, 12, 14, 16, 18, 89 or 20 or a functional variant or homologue thereof;
- and
- wherein the tissue regeneration factor precursor is selected from SEQ ID NO:
- 22 or a functional variant or homologue thereof, wherein the homologue is selected from SEQ ID NO:
- 24, 26, 28, 30, 32, 34, 36, 38, 91 or 40 or a functional variant or homologue thereof;
- and
- wherein the tissue regeneration factor receptor is selected from SEQ ID NO:
- 42 or a functional variant or homologue thereof, wherein the homologue is selected from SEQ ID NO:
- 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 93 or 78 or a functional variant or homologue thereof.
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A genetically altered plant, plant part thereof, or plant cell, wherein said plant, part thereof or plant cell is characterized by increased expression and/or levels at least one of
- a tissue regeneration factor;
- and/or
- a tissue regeneration factor precursor;
- and/or
- iii a tissue regeneration factor receptor
- wherein the tissue regeneration factor is selected from SEQ ID NO:
- 2 or functional variant or homologue thereof, wherein the homologue is selected from SEQ ID NO:
- 4, 6, 8, 10, 12, 14, 16, 18, 89 or 20 or a functional variant or homologue thereof;
- and
- wherein the tissue regeneration factor precursor is selected from SEQ ID NO:
- 22 or a functional variant or homologue thereof, wherein the homologue is selected from SEQ ID NO:
- 24, 26, 28, 30, 32, 34, 36, 38, 91 or 40 or a functional variant or homologue thereof;
- and
- wherein the tissue regeneration factor receptor is selected from SEQ ID NO:
- 42 or a functional variant or homologue thereof, wherein the homologue is selected from SEQ ID NO:
- 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 93 or 78 or a functional variant or homologue thereof;
- or
- wherein said plant is characterized by one or more mutation in the plant genome, where the mutation is the insertion of at least one additional copy of a nucleic sequence encoding a tissue regeneration factor precursor and/or at least one additional copy of a nucleic acid sequence encoding a tissue regeneration factor receptor, such that said nucleic acid sequence(s) is operably linked to a regulatory sequence, and wherein preferably the mutation is introduced using targeted genome editing;
- wherein the tissue regeneration factor precursor is selected from SEQ ID NO:
- 22 or a functional variant or homologue thereof, wherein the homologue is selected from SEQ ID NO:
- 24, 26, 28, 30, 32, 34, 36, 38, 91 or 40 or a functional variant or homologue thereof;
- and
- wherein the tissue regeneration factor receptor is selected from SEQ ID NO:
- 42 or a functional variant or homologue thereof, wherein the homologue is selected from SEQ ID NO:
- 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 93 or 78 or a functional variant or homologue thereof.
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Dependencies are inferred from the claim text.