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US 2026/0265707 A1Published application
Modified transglutaminase
AssigneeAmano Enzyme Inc.
InventorKazunori Yoshida
Life of this patent
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Abstract
An object of the present invention is to find a new mutation effective for improving transglutaminase, and to provide a highly useful modified transglutaminase. Disclosed is a highly useful modified transglutaminase having an amino acid substitution that results in a reduction in temperature stability, an improvement in heat resistance, an improvement in oxidation resistance, an improvement in reactivity, or conversion into deamidase.
Claims9
A modified transglutaminase,
- wherein the modified transglutaminase exhibits a property change corresponding to the amino acid substitution,
- the property change due to the amino acid substitution is a reduction in temperature stability,
- the modified transglutaminase has an amino acid sequence comprising any one of the following amino acid substitutions (1) to (12) in the sequence of SEQ ID NO:
- 1 or an amino acid sequence showing 80% or more identity to the amino acid sequence, provided that a difference in amino acid sequence occurs at a portion other than the position of the amino acid substitution:
- (1) an amino acid substitution in which the mutation point is V6, the amino acid after substitution is Q, I, M, S, C, K, L, H, F, G, N, P, R, W or Y;
- (2) an amino acid substitution in which the mutation point is L60, the amino acid after substitution is I, M, V, A, C, E, F, Q, S, T, W or Y;
- (3) an amino acid substitution in which the mutation point is V67, the amino acid after substitution is L, N, A, C, M, Q or S;
- (4) an amino acid substitution in which the mutation point is F85, the amino acid after substitution is M;
- (5) an amino acid substitution in which the mutation point is F90, the amino acid after substitution is C, M, H, L or V;
- (6) an amino acid substitution in which the mutation point is F108, the amino acid after substitution is R, L, T, A, I, K, N or V;
- (7) an amino acid substitution in which the mutation point is F117, the amino acid after substitution is M or L;
- (8) an amino acid substitution in which the mutation point is W203, the amino acid after substitution is F;
- (9) an amino acid substitution in which the mutation point is T273, and the amino acid after substitution is I, V, M, C, S, L, R, G, A, E, F, Y, D, K, W or H;
- (10) an amino acid substitution in which the mutation point is N276, the amino acid after substitution is C, E, K, L, S, T or V;
- (11) an amino acid substitution in which the mutation point is Y310, the amino acid after substitution is C, M or I;
- and
- (12) an amino acid substitution in which the mutation point is L60, the amino acid after substitution is I.
14 more elements
Dependencies are inferred from the claim text.