Loading patent…
AssigneesAgricultural Genomics Institute At Shenzhen, Chinese Academy of Agricultural Sciences, Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences
InventorsJianbin YAN, Sanwen HUANG, Bin JIANG
Estimating the term…
Disclosed is a bioenzyme for catalyzing formation of a three-membered or four-membered oxygen heterocyclic structure of a taxane molecule, and gene, biomaterial and use thereof. The bioenzyme includes: at least one of amino acid sequences represented by SEQ ID NO: 25; a fusion protein thereof; a mutant protein thereof; and a homologous protein thereof. The bioenzyme provided by the disclosure can be catalyzed to form a three-membered and/or four-membered oxygen heterocyclic structure of taxane molecule, so that a new gene resource is provided for improving the yield of related compounds with an oxetane ring group and an oxirane ring group (such as paclitaxel) and completing a synthetic pathway of the related compounds.
A recombinant DNA vector comprising a heterologous nucleic acid sequence, wherein the nucleic acid sequence encodes a protein named TOT (Taxane C4-C20 oxetane-forming enzyme) comprising a sequence having 64.8% or more identity to SEQ ID NO:
A transgenic plant, plant part, or plant cell having taxane C4-C20 oxetane-forming enzyme activity, wherein the activity is conferred by a transgene comprising a nucleic acid sequence encoding a TOT protein comprising a sequence having 64.8% or more identity to SEQ ID NO:
Dependencies are inferred from the claim text.