Recombinant polypeptides for biosynthesis of thymohydroquinone (thq)
AssigneeEpimeron USA, Inc.
InventorsTrish Choudhary, Matthew Workentine, Christian Hobson, Michal Pyc
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Abstract
The present disclosure relates to recombinant polypeptides with cytochrome P450 (CYP) activity capable of selectively catalyzing the conversion of precursor compounds, carvacrol and/or thymol, to the product compound, thymohydroquinone (THQ), and recombinant host cells that heterologously express genes encoding these polypeptides (e.g., in yeast) and that can be used for enhanced fermentative bioproduction of the product compound THQ.
Claims27
A recombinant host cell comprising a heterologous nucleic acid encoding a polypeptide with CYP activity capable of converting carvacrol and/or thymol to THQ, wherein the polypeptide comprises an amino acid sequence of at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.5% sequence identity to a sequence selected from the group consisting of SEQ ID NO:
- 24, 8, 10, 12, 14, 16, 18, 20, 22, 26, 28, 30, 32, 34, and 36.
A polynucleotide comprising a sequence of at least 80% identity to a sequence selected from the group consisting of SEQ ID NO:
- 23, 7, 9, 11, 13, 15, 17, 19, 21, 25, 27, 29, 31, 33, and 35.
A method for preparing compound (1a) or a derivative of compound (1a)
- comprising contacting under suitable reactions conditions compound (2a) and/or compound (2b) or a derivative of compound (2a) and/or a derivative of compound (2b)
- with a recombinant polypeptide with CYP activity, wherein the recombinant polypeptide with CYP activity comprises an amino acid sequence of at least 80% sequence identity to a sequence selected from the group consisting of SEQ ID NO:
- 24, 8, 10, 12, 14, 16, 18, 20, 22, 26, 28, 30, 32, 34, and 36.
The method of any one of claims 16-18, wherein the contacting with the polypeptide with CYP activity occurs in the presence of host cell that expresses the polypeptide.
A composition comprising:
- (a) a recombinant polypeptide with CYP activity comprising an amino acid sequence of at least 80% sequence identity to a sequence selected from the group consisting of SEQ ID NO:
- 24, 8, 10, 12, 14, 16, 18, 20, 22, 26, 28, 30, 32, 34, and 36;
- and
- (b) a compound (2a) and/or compound (2b) or a derivative of compound (2a) and/or a derivative of compound (2b)
Dependencies are inferred from the claim text.