Compositions and methods of preparing airway cells
AssigneeYale University
InventorsMahboobe GHAEDI, Laura NIKLASON
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Abstract
The present invention provides compositions and methods of preparing airway cells. In one aspect, an epithelial airway cell derived from an induced pluripotent stem (iPS) cell characterized by expression of airway cell surface markers and an ability to proliferate is described. In another aspect, methods of differentiating an iPS into an epithelial airway cell is provided. Engineered lungs, methods of making such engineered lungs comprising the epithelial airway cells and treating respiratory disorders are also disclosed.
Claims23
1-32. (canceled)
A method of improving, treating or relieving a symptom of a respiratory disorder in a subject in need thereof, the method comprising administering a therapeutically effective amount of a pharmaceutical formulation comprising airway epithelial cells derived from induced pluripotent stem (iPS) cells and at least one pharmaceutically acceptable carrier,
- wherein the airway epithelial cells are characterized by:
- expression of airway cell surface markers comprising Clara cell secretory protein (CCSP), cytokeratin 5 (KRT5), and FOXJ1;
- and
- an ability to proliferate in culture without loss of the airway cell surface markers.
An engineered three-dimensional lung tissue comprising airway epithelial cells derived from induced pluripotent stem (iPS) cells and a three-dimensional scaffold,
- wherein the airway epithelial cells are characterized by:
- expression of airway cell surface markers comprising Clara cell secretory protein (CCSP), cytokeratin 5 (KRT5), and FOXJ1;
- and
- an ability to proliferate in culture without loss of the airway cell surface markers.
Dependencies are inferred from the claim text.