US 2026/0265696 A1 · HUMAN IPSC DERIVED AMELOBLASTS AND USES THEREOF

Abstract

Enamel forms during early development, before the tooth breaks through the gum, and it covers the visible portion of the tooth. During development, epithelial cells within the enamel organ differentiate into ameloblasts, which produce the hardened, high mineral-content enamel. The technology as described herein relates to discovery of methods for generating ameloblasts or organoids thereof in vitro and compositions that utilize the generated ameloblasts for restoring enamel products.

Claims30

Dependent claims

A method of preparing an ameloblast culture, the method comprising, in order,

a) contacting, in culture, an induced pluripotent stem cell (iPSC) with an activator of the Hedgehog pathway;
b) adding Bone Morphogenetic Protein 4 (BMP4) to the culture of (a);
c) adding an inhibitor of BMP type I receptors, a Wnt activator, Epidermal Growth Factor (EGF) and Neurotrophin-4 (NT4) to the culture of (b) and incubating to form oral epithelium cells;
and
d) adding BMP4 and transforming growth factor β1 (TGF-μ1) to the culture of (c) and incubating to form ameloblasts.

2 more elements

Dependencies are inferred from the claim text.

Human ipsc derived ameloblasts and uses thereof

Life of this patent

Human ipsc derived ameloblasts and uses thereof

Life of this patent

Abstract

Claims30