Cell treatment product preparation process and use thereof
AssigneeChongqing Precision Biotech Company Limited
InventorsLinling Wang, Zhi Yang, Yunyan Li, Qianzhen Zhang, Meiling Wang, Wei Zhu, Jun Chen, Yi Huang, Jiao Zheng, Junjie Shen, Juan Hong
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Abstract
The present application provides a method for preparing a cell population expressing a chimeric antigen receptor (CAR), and a composition, a culture medium or a kit applicable to the method. The cell population has reduced in vivo cytotoxicity.
Claims31
A method of preparing a cell population expressing a chimeric antigen receptor (CAR), comprising:
- step (i):
- contacting the cell population with a CD3/TCR complex agonist;
- step (ii):
- introducing a nucleic acid molecule encoding the CAR into the cell population via transduction, thereby providing cells containing the nucleic acid molecule, and
- step (iii):
- culturing the cell population for a period of time, and harvesting the cell population.
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A composition for preparing a cell population expressing a chimeric antigen receptor (CAR), comprising a CD3/TCR complex agonist and an immune cell-activating cytokine, wherein the immune cell-activating cytokine excludes or comprises IL-2.
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A cell culture medium for preparing a cell population expressing a chimeric antigen receptor (CAR), comprising a CD3/TCR complex agonist and an immune cell-activating cytokine, wherein the immune cell-activating cytokine excludes IL-2.
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Dependencies are inferred from the claim text.