Biohybrid robot including retinal/brain organoid, motor nerve spheroid, and muscle bundle, and manufacturing method therefor

A method for fabricating a biohybrid robot, comprising the following steps:

• a conjugation step of producing a conjugate by conjugating a retinal organoid and a brain organoid;
• a first connection step of connecting a motor neuron spheroid to the conjugate;
• a second connection step of connecting a muscle bundle to the motor neuron spheroid connected to the conjugate;
• and
• a third connection step of connecting a robot structure to the muscle bundle connected to the conjugate.

A method for screening a candidate substance for the treatment of a neurodegenerative disease or eye disease, comprising the following steps:

• a motor system preparation step of preparing a biohybrid robot comprising a conjugate of a retinal organoid and a brain organoid, a motor neuron spheroid connected to the conjugate, a muscle bundle connected to the motor neuron spheroid and a robot structure connected to the muscle bundle;
• a drug contact step of contacting the candidate substance to the biohybrid robot;
• and
• a drug evaluation step of comparing the degree of contraction induced by light stimulation in the muscle bundle after contact with the candidate substance to that in the muscle bundle not contacted with the candidate substance.