Anti-cd40 antibody and use thereof

An antibody or antigen-binding fragment thereof capable of specifically binding to CD40, wherein the antibody or antigen-binding fragment thereof comprises:

• (1) the following three complementary determining regions (CDR) of a heavy chain variable region (VH):
• (a) a VH CDR1, having a structure selected from the group consisting of:
• (SEQ ID NO:
• 76)
• FTFX1X2YX3MH,
• (SEQ ID NO:
• 6)
• GSISSYYWS;
• (b) a VH CDR2, having a structure selected from the group consisting of:
• (SEQ ID NO:
• 77)
• VIX4YX5X6X7X8KYYAX9SVKG,
• (SEQ ID NO:
• 78)
• GISWSSX10SIX11YADSVKG,
• (SEQ ID NO:
• 22)
• SIYYSGSTNYNPSLKS;
• (c) a VH CDR3, having a structure selected from the group consisting of:
• (SEQ ID NO:
• 23)
• ARDTSRGHDI,
• (SEQ ID NO:
• 24)
• AKDPTATTGARGYFDL,
• (SEQ ID NO:
• 25)
• RDANYYKWHPY;
• and/or,
• (2) the following three complementary determining regions (CDR) of a light chain variable region (VL):
• (d) a VL CDR1, having a structure selected from the group consisting of:
• (SEQ ID NO:
• 79)
• X12ASQSVSX13X14YLA,
• (SEQ ID NO:
• 7)
• X15ASX16X17ISSWLA;
• (e) a VL CDR2, having a structure selected from the group consisting of:
• (SEQ ID NO:
• 8)
• GASX18X19X20X21,
• (SEQ ID NO:
• 9)
• AASX22LX23S;
• (f) a VL CDR3, having a structure selected from the group consisting of:
• (SEQ ID NO:
• 10)
• X24X25YX26DYPPFT,
• (SEQ ID NO:
• 11)
• QQX27X28SX29PPT;
• wherein,
• X1 is selected from the group consisting of (i) amino acid residues S, E, G, D and (ii) amino acid residues that are conservative substitutions relative to (i);
• X2 is selected from the group consisting of (i) amino acid residues S, K, D, T and (ii) amino acid residues that are conservative substitutions relative to (i);
• X3 is selected from the group consisting of (i) amino acid residues G, A and (ii) amino acid residues that are conservative substitutions relative to (i);
• X4 is selected from the group consisting of (i) amino acid residues S, H and (ii) amino acid residues that are conservative substitutions relative to (i);
• X5 is selected from the group consisting of (i) amino acid residues E, H and (ii) amino acid residues that are conservative substitutions relative to (i);
• X6 is selected from the group consisting of (i) amino acid residues G, A and (ii) amino acid residues that are conservative substitutions relative to (i);
• X7 is selected from the group consisting of (i) amino acid residues S, E, T, V and (ii) amino acid residues that are conservative substitutions relative to (i);
• X8 is selected from the group consisting of (i) amino acid residues N, S, I, K and (ii) amino acid residues that are conservative substitutions relative to (i);
• X9 is selected from the group consisting of (i) amino acid residues D, E and (ii) amino acid residues that are conservative substitutions relative to (i);
• X10 is selected from the group consisting of (i) amino acid residues G, D and (ii) amino acid residues that are conservative substitutions relative to (i);
• X11 is selected from the group consisting of (i) amino acid residues G, H and (ii) amino acid residues that are conservative substitutions relative to (i);
• X12 is selected from the group consisting of (i) amino acid residues R, Q and (ii) amino acid residues that are conservative substitutions relative to (i);
• X13 is selected from the group consisting of (i) amino acid residues Y, S and (ii) amino acid residues that are conservative substitutions relative to (i);
• X14 is selected from the group consisting of (i) amino acid residues S, D, N and (ii) amino acid residues that are conservative substitutions relative to (i);
• X15 is selected from the group consisting of (i) amino acid residues R, E and (ii) amino acid residues that are conservative substitutions relative to (i);
• X16 is selected from the group consisting of (i) amino acid residues Q, K and (ii) amino acid residues that are conservative substitutions relative to (i);
• X17 is selected from the group consisting of (i) amino acid residues G, V and (ii) amino acid residues that are conservative substitutions relative to (i);
• X18 is selected from the group consisting of (i) amino acid residues S, T, A and (ii) amino acid residues that are conservative substitutions relative to (i);
• X19 is selected from the group consisting of (i) amino acid residues R, L and (ii) amino acid residues that are conservative substitutions relative to (i);
• X20 is selected from the group consisting of (i) amino acid residues N, A and (ii) amino acid residues that are conservative substitutions relative to (i);
• X21 is selected from the group consisting of (i) amino acid residues T, N and (ii) amino acid residues that are conservative substitutions relative to (i);
• X22 is selected from the group consisting of (i) amino acid residues S. Y and (ii) amino acid residues that are conservative substitutions relative to (i);
• X23 is selected from the group consisting of (i) amino acid residues E, Q and (ii) amino acid residues that are conservative substitutions relative to (i);
• X24 is selected from the group consisting of (i) amino acid residues S, G, Q and (ii) amino acid residues that are conservative substitutions relative to (i);
• X25 is selected from the group consisting of (i) amino acid residues E, Q, D and (ii) amino acid residues that are conservative substitutions relative to (i);
• X26 is selected from the group consisting of (i) amino acid residues A, S and (ii) amino acid residues that are conservative substitutions relative to (i);
• X27 is selected from the group consisting of (i) amino acid residues R, H, V and (ii) amino acid residues that are conservative substitutions relative to (i);
• X28 is selected from the group consisting of (i) amino acid residues S, L, A and (ii) amino acid residues that are conservative substitutions relative to (i);
• X29 is selected from the group consisting of (i) amino acid residues F. Y and (ii) amino acid residues that are conservative substitutions relative to (i).