Conditionally activated receptor signaling by using scaffold protein

A method for producing a protein complex, wherein

• the protein complex comprises a first binding domain, a second binding domain, a third binding domain and a fourth binding domain, wherein the first binding domain is capable of binding to a scaffold protein, the second binding domain is capable of binding to a first receptor protein, the third binding domain is capable of binding to the scaffold protein, and the fourth binding domain is capable of binding to a second receptor protein,
• wherein the first binding domain and the third binding domain are capable of non-competitively binding to the scaffold protein,
• wherein the first receptor protein and the second receptor protein are each receptor subunits that can associate to form a receptor complex, and
• wherein the protein complex is capable of inducing receptor signaling of the receptor complex,
• the method comprising the steps of:
• (i) Identifying a first binding domain and a third binding domain capable of non-competitively binding to the scaffold protein,
• (ii) connecting, optionally via a polynucleotide encoding a linker, a polynucleotide encoding the first binding domain with a polynucleotide encoding the second binding domain,
• (iii) connecting, optionally via a polynucleotide encoding a linker, a polynucleotide encoding the third binding domain with a polynucleotide encoding the fourth binding domain, and
• (iv) expressing the polynucleotides of (ii) and (iii) to produce the protein complex.