Use of molecules isolated from bacteria present in digestate produced by biogas plants as immunostimulants of plant defence responses
AssigneesUniversita' Degli Studi Di Roma “La Sapienza”, Agrolio Srl
InventorsVincenzo LIONETTI, Savino AGRESTI, Giulia DE LORENZO, Marco GRECO
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Abstract
A process is described for the valorisation of the microbial fraction contained in the waste material from biogas plants following anaerobic digestion of olive pomace, thanks to which an extract enriched in proteins and peptides of microbial origin is obtained for use as a plant protection product in agricultural production.
Claims10
A process for the treatment of a digestate derived from an anaerobic digestion of an olive pomace comprising the following steps:
- (a) undergoing or subjecting the digestate derived from the anaerobic digestion of the olive pomace to sedimentation to obtain a liquid fraction and a solid fraction;
- (b) sampling the liquid fraction obtained at the end of step a) and centrifugation of the liquid fraction to obtain a liquid supernatant fraction and a solid pellet of bacterial origin;
- (c) resuspending the solid pellet of bacterial origin as obtained at the end of step b);
- (d) undergoing the resuspended solid pellet of bacterial origin as obtained at the end of step c) to mechanical cellular breaking by sonication to obtain a solution;
- and
- (e) additional centrifugation of the solution obtained at the end of step d) to obtain a cellular residue fraction and a liquid phase as final product,
- wherein the final product is a not-infectious, bacterial-protein and bacterial-peptides enriched solution.
A not-infectious, bacterial-protein and bacterial-peptides enriched solution obtained by a process comprising the following steps:
- (a) sedimentation of a digestate derived from an anaerobic digestion of an olive pomace to obtain a liquid fraction and a solid fraction;
- (b) sampling the liquid fraction obtained at the end of step a) and centrifugation of the liquid fraction to obtain a liquid supernatant fraction and a solid pellet fraction, wherein the pellet is of bacterial origin;
- (c) resuspending the solid pellet of bacterial origin as obtained at the end of step b);
- (d) undergoing the resuspended solid pellet of bacterial origin as obtained at the end of step c) to mechanical cellular breaking by sonication to obtain a solution;
- and
- (e) additional centrifugation of the solution obtained at the end of step d) to obtain a cellular residue fraction and a liquid phase being the solution as final product.
The process of claim 7, wherein the bacterial protein PAMPS fraction is in an amount ranging from 10 to 500 μg/ml.
Dependencies are inferred from the claim text.