Apparatus and method for separating and concentrating a component of a fluid

A device to separate and concentrate a component of a whole material, comprising:

• a separation container forming a first portion of the device and a concentration container forming a second portion of the device;
• a buoy system positioned within a first chamber of the separation container, wherein the buoy system configured to assist in a separation of the whole material;
• a first port assembly and a second port assembly in fluid communication with the first chamber;
• a third port assembly in fluid communication with a second chamber of the concentration container;
• a vent assembly including a pathway configured to a provide a flow of air between the first chamber and the second chamber;
• a fourth port assembly in fluid communication with a third chamber of the concentration container;
• and
• a filter separating the third chamber and the second chamber.

A method of processing a biological material, comprising:

• introducing the biological material including a whole blood through a first port assembly into a first chamber of a separation container of a device having an assembly that includes the separation container and a concentration container;
• centrifuging the device to separate the whole blood into fractions;
• withdrawing a platelet poor plasma of the fractions through the first port assembly from the first chamber;
• withdrawing a platelet rich plasma of the fractions through a second port assembly from between buoy members in the first chamber;
• introducing with a third port assembly the platelet rich plasma into a second chamber of the concentration container;
• centrifuging the device to concentrate the platelet rich plasma;
• and
• withdrawing the platelet rich plasma once concentrated from a third chamber of the concentration container through a fourth port assembly.