Production and use of extracellular vesicle-contained enampt
AssigneeWashington University
InventorsShin-Ichiro Imai, Mitsukuni Yoshida
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Abstract
The present invention relates to various compositions comprising NAMPT and/or mutant thereof, processes for preparing these compositions, and various methods of using these compositions to prevent or treat an age-associated condition in a subject. The present invention also relates to methods of increasing NMN and/or NAD+ biosynthesis in a subject or in a cell.
Claims22
-37. (canceled)
A method of increasing NMN and/or NAD+ biosynthesis in a subject, the method comprising administering to the subject a composition comprising extracellular nicotinamide phosphoribosyltransferase (eNAMPT) and/or mutant thereof and an extracellular vesicle.
A method of preventing or treating an age-associated condition in a subject, the method comprising administering to the subject a composition comprising extracellular nicotinamide phosphoribosyltransferase (eNAMPT) and/or mutant thereof and an extracellular vesicle.
A method of increasing NMN and/or NAD+ biosynthesis in a cell, the method comprising applying a composition comprising extracellular nicotinamide phosphoribosyltransferase (eNAMPT) and/or mutant thereof and an extracellular vesicle to the cell.
A process for preparing a composition comprising extracellular nicotinamide phosphoribosyltransferase (eNAMPT) and/or mutant thereof and an extracellular vesicle, the process comprising:
- subjecting a medium comprising vesicles comprising the extracellular vesicle and eNAMPT and/or mutant thereof to a separation process to obtain an enriched vesicle fraction, wherein the concentration of the vesicles in the enriched vesicle fraction is greater than the concentration of the vesicles in the medium.
(canceled)
Dependencies are inferred from the claim text.