Methods of analyzing recombinant polyclonal proteins
AssigneeGigaGen, Inc.
InventorsHongjun YUE, Rena Aviva MIZRAHI, Ariel Reed NIEDECKEN, Yao Yuan CHIANG
Life of this patent
Estimating the term…
Abstract
Provided herein is a method for analyzing and characterizing compositions comprising recombinant polyclonal proteins (ABPs). Also provided are use of the method related to production, manufacture, distribution, storage and therapeutic use of the ABPs.
Claims34
A method of analyzing a test library comprising antigen binding proteins (ABPs), comprising:
- (a) measuring a distribution of charge states of the test library, wherein the test library comprises at least 100 ABPs;
- (b) comparing the distribution of charge states with a reference distribution, wherein the reference distribution is a distribution of charge states of a reference library;
- and
- (c) determining a quality of the test library based on the comparison.
4-12. (canceled)
(canceled)
18-24. (canceled)
(canceled)
(canceled)
30-31. (canceled)
33-35. (canceled)
(canceled)
42-43. (canceled)
46-48. (canceled)
A method of analyzing a test library comprising antigen binding proteins (ABPs), comprising:
- (a) measuring size heterogeneity of the test library, wherein the test library comprises at least 100 ABPs;
- (b) comparing peaks corresponding to high molecular weight species (HMW), polyclonal antibody monomers (pAb peaks), and low molecular weight species (LMW);
- and
- (c) determining a quality of the test library based on the comparison.
51-55. (canceled)
Dependencies are inferred from the claim text.