Back to results
US 2026/0259211 A1Published application
Isolation of fetal cells using facs
AssigneeArcedi Biotech ApS
InventorsBolette Hestbek Nicolaisen, David Mathias Martin Leiding Kolvraa, Inga Baasch Christensen, Katarina Ravn, Line Dahl Jeppesen, Lotte Hatt, Palle Schelde Jensen, Ripudaman Singh
Life of this patent
Estimating the term…
Abstract
The present invention relates to automated methods for isolating fetal cells from a sample, such as a blood sample, derived from a pregnant woman. The isolated fetal cells can be used for identifying genetic abnormalities in the fetal DNA.
Claims30
A method of prenatal diagnostics comprising the steps of:
- A. providing a blood sample from a woman carrying a fetus;
- B. contacting cells comprised in said blood sample with at least one magnetic labelling agent directed against a fetal trophoblast marker;
- C. enriching said sample for fetal trophoblasts with magnetic activated cell sorting (MACS);
- D. following the enrichment step, the single cell sorting by FACS is performed by contacting the enriched cells with at least one fluorescent labelling agent selected from each of the groups:
- i. fluorescent labelling agent directed against the nucleus,
- ii. fluorescent labelling agent directed against a maternal cell marker, and
- iii. fluorescent labelling agent directed against a fetal trophoblast marker;
- E. single cell sorting the fluorescently labelled cells on a fluorescence activated cell sorter (FACS) from said enriched sample based on:
- iv. positive selection of said fluorescent labelling agent directed against a fetal trophoblast marker,
- v. positive selection of said fluorescent labelling agent directed against the nucleus, and
- vi. negative selection of said fluorescent labelling agent directed against a maternal cell marker;
- F. identifying fetal trophoblasts among said sorted cells, comprising a step of assigning a fetal origin classifier to individually sorted cells by obtaining a genotype from the single sorted cells;
- and
- G. diagnosing the phenotype of the fetus.
11 more elements
A method of prenatal diagnostics comprising the steps of:
- A. providing a blood sample from a woman carrying a fetus,
- B. contacting cells comprised in said blood sample with at least one fluorescent labelling agent selected from each of the groups:
- i. fluorescent labelling agent directed against the nucleus;
- ii. fluorescent labelling agent directed against a maternal cell marker;
- and
- iii. fluorescent labelling agent directed against a fetal trophoblast marker
- C. enriching said sample for fetal trophoblasts on a fluorescence activated cell sorter (FACS) based on fluorescence from said fluorescent labelling agents,
- D. following the enrichment step, the single cell sorting by FACS is performed by single cell sorting the fluorescently labelled cells on a FACS from said enriched sample based on:
- iv. positive selection of said fluorescent labelling agent directed against a fetal trophoblast marker,
- v. positive selection of said fluorescent labelling agent directed against the nucleus, and
- vi. negative selection of said fluorescent labelling agent directed against a maternal cell marker;
- E. identifying trophoblasts among said sorted cells, comprising a step of assigning a fetal origin classifier to individually sorted cells by obtaining a genotype from said fetal trophoblast;
- and
- F. diagnosing the phenotype of the fetus.
11 more elements
Dependencies are inferred from the claim text.