Compositions and methods for detection of neisseria gonorroheae

A method of detecting Neisseria Gonorroheae (NG) in a sample, the method comprising:

• performing an amplifying step comprising contacting the sample with a set of NG PivNg gene primers to produce an amplification product if NG PivNg nucleic acid is present in the sample;
• performing a hybridizing step comprising contacting the amplification product with one or more detectable NG PivNg gene probes;
• and
• detecting the presence or absence of the amplification product, wherein the presence of the amplification product is indicative of the presence of NG in the sample and wherein the absence of the amplification product is indicative of the absence of NG in the sample;
• wherein the set of NG PivNg gene primers comprise a first primer comprising a first oligonucleotide sequence of SEQ ID NOs:
• 1, or a complement thereof, and a second primer comprising a second oligonucleotide sequence of SEQ ID NOs:
• 2, or a complement thereof;
• and
• wherein the one or more detectable NG PivNg gene probes comprises a third oligonucleotide sequence of SEQ ID NOs:
• 3, or the complement thereof.