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AssigneesThe Broad Institute, Inc., Massachusetts Institute of Technology
InventorsXiao Wang, Hu Zeng, Jingyi Ren, Jiakun Tian, Jianting Guo
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The present disclosure provides methods, compositions, and systems for profiling epitranscriptomic RNA modifications in a cell. The present disclosure also provides methods for profiling interactions between one or more RNAs of interest in a cell and an RNA-binding protein (e.g., a protein that introduces an epitranscriptomic modification on an RNA of interest). Also provided by the present disclosure are methods for diagnosing a disease or disorder in a subject based on a profile of epitranscriptomic RNA modifications or a profile of interactions between an RNA binding protein and RNAs in a cell, including cells within an intact tissue. Methods of screening for or testing a candidate agent capable of modulating epitranscriptomic modification of one or more RNAs or interactions between one or more RNAs and an RNA-binding protein are also provided by the present disclosure. The present disclosure also provides methods for treating a disease or disorder in a subject in need thereof. Pairs of probes and sets of probes comprising oligonucleotide portions, which may be useful for performing the methods described herein, are also described by the present disclosure. Additionally, the present disclosure provides kits comprising any of the probes described herein.
A method for profiling epitranscriptomic RNA modifications in a cell, the method comprising:
A method for profiling epitranscriptomic RNA modifications in a cell, the method comprising:
A method for profiling interactions between an RNA-binding protein and one or more RNAs of interest in a cell, the method comprising:
A method for diagnosing a disease or disorder in a subject, the method comprising:
A method for diagnosing a disease or disorder in a subject, the method comprising:
A method for diagnosing a disease or disorder in a subject, the method comprising:
A method for screening for an agent capable of modulating epitranscriptomic modification of one or more RNAs, the method comprising:
A method for screening for an agent capable of modulating epitranscriptomic modification of one or more RNAs, the method comprising:
A method for screening for an agent capable of modulating interactions between RNA and RNA-binding proteins, the method comprising:
A method for treating a disease or disorder in a subject, the method comprising:
A method for treating a disease or disorder in a subject, the method comprising:
A method for treating a disease or disorder in a subject, the method comprising:
A pair of probes comprising a first probe and a second probe, wherein:
A set of probes comprising a first probe, a second probe, and a third probe, wherein:
A set of probes comprising a first probe, a second probe, and a third probe, wherein:
A system for profiling epitranscriptomic RNA modifications in a cell comprising
A system comprising:
A system comprising:
A probe comprising a portion that recognizes an epitranscriptomic RNA modification and an oligonucleotide portion that is complementary to a portion of another probe.
Dependencies are inferred from the claim text.