ANALYTE QUANTIFICATION WITH BEAD AND IMMUNO qPCR-BASED ELISA (BIQ-ELISA)
OwnerYing-Qing Mao, Ruo-Pan Huang +2inventors
InventorsYing-Qing Mao, Ruo-Pan Huang, Tao Wang, Xuelin Wang
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Abstract
Disclosed are kits and methods for detecting target analytes in a sample using modified magnetic particles and nucleic acid barcodes. The kits include magnetic particles conjugated to a first binding agent and a second binding agent conjugated to a nucleic acid barcode, with the magnetic particles and binding agents along with target antigens form immune complex. The nucleic acid barcode is amplified using qPCR and/or sequenced using Next Generation Sequencing (NGS). Also disclosed are methods for forming immune complexes, performing signal amplification, and determining analyte concentrations.
Claims20
A kit for detection of a target analyte in a sample, the kit comprising:
- (a) a first reagent;
- and
- (b) a second reagent,
- wherein:
- (i) the first reagent comprises magnetic particles modified with a first binding agent specific for the analyte and
- (ii) the second reagent comprises a second binding agent specific for the analyte, conjugated to a nucleic acid barcode.
A kit for detection of one or more target analyte(s) in a sample, the kit comprising:
- (a) a first reagent;
- and
- (b) a second reagent,
- wherein:
- (i) the first reagent comprises magnetic particles modified with a member of an affinity binding pair, and
- (ii) the second reagent comprises a second binding agent specific to the analyte and a nucleic acid barcode, wherein the second binding agent is conjugated to the nucleic acid barcode.
Dependencies are inferred from the claim text.