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US 2026/0258473 A1Published application
Fluorescent probe for detection of enpp activity
AssigneesThe University of Tokyo, Public University Corporation Nagoya City University, Riken
InventorsYasuteru URANO, Toru KOMATSU, Shingo SAKAMOTO, Hiroyuki NOJI, Hidehiko NAKAGAWA, Mitsuyasu KAWAGUCHI, Rikiya WATANABE
Life of this patent
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Abstract
A novel fluorescent probe that includes a compound of the following general formula (II) or a salt thereof:
Claims14
A compound of formula (II) or a salt thereof:
- wherein
- R1a is one or two monovalent substituents present on a benzene ring, which are electron donating groups, and when a plurality of R1as are present, R1as are the same or different;
- R2a is one or two monovalent substituents present on a benzene ring, which have an anionic functional group at an end, and when a plurality of R2as are present, R2as are the same or different;
- R3a and R4a are each independently a hydrogen atom, an alkyl group having 1 to 6 carbon atoms, or a halogen atom;
- R5a and R6a are each independently an alkyl group having 1 to 6 carbon atoms, or an aryl group;
- R7a and R8a are each independently a hydrogen atom, an alkyl group having 1 to 6 carbon atoms, or a halogen atom;
- Za is an oxygen atom or N+R9aR10a,
- where R9a and R10a are each independently a hydrogen atom, or an alkyl group having 1 to 10 carbon atoms,
- R9a and R10a are optionally linked together to form a four- to seven-membered heterocyclyl containing a nitrogen atom bonded to R9a and R10a, and
- R9a or R10a, or each of both R9a and R10a is optionally linked with R3a or R7a to form a five- to seven-membered heterocyclyl or heteroaryl containing a nitrogen atom bonded to R9a or R10a, or optionally contains one to three additional hetero atoms selected from the group consisting of an oxygen atom, a nitrogen atom and a sulfur atom as ring forming members, and the heterocyclyl or heteroaryl is optionally substituted with an alkyl having 1 to 6 carbon atoms, an alkenyl having 2 to 6 carbon atoms, an alkynyl having 2 to 6 carbon atoms, an aralkyl group having 6 to 10 carbon atoms, or an alkyl-substituted alkenyl group having 6 to 10 carbon atoms;
- Ya is a single bond, —O—(CH2)n1—, —O—(CH2)n2—Ar1—, —NH—(CH2)n3— or —NH—(CH2)n4—Ar2—,
- where n1, n2, n3 and n4 are each independently an integer of 1 to 10, and
- Ar1 and Ar2 are each independently a substituted or unsubstituted arylene group;
- and
- (Nu) is represented by R—S—
- where R is an organic base, and
- S represents a sugar or a partial structure of a derivative thereof, or a single bond.
14 more elements
A method for detecting the enzyme activity of ENPP3, comprising bringing ENPP3 into contact with the following compound (A) and/or the following compound (B)
Dependencies are inferred from the claim text.