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US 2026/0258455 A1Published application
Unique method to trigger lactic acid production in streptomyces coelicolor a3(2)
AssigneeGebze Teknik Universitesi
InventorsNagihan GENEL, Sedef TUNCA GEDIK
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Abstract
A method to trigger lactic acid production in Streptomyces coelicolor A3(2) is provided. In the present invention, the morphology and metabolism of the recombinant Streptomyces coelicolor A3(2) cell totally changed because of using “aadA gene” as a selective marker. After various experiments with different controls it was realized that this change is coming only from the expression of “aadA gene” itself.
Claims3
A method to trigger a lactic acid production in Streptomyces coelicolor A3(2), comprising the following process steps:
- amplifying Streptomycin resistance gene (aadA) by a polymerase chain reaction (PCR) using pIJ778 plasmid as a template,
- cloning the Streptomycin resistance gene (aadA) into an integrative and conjugative pRA plasmid to obtain a recombinant pRAstr plasmid,
- transferring the recombinant pRAstr plasmid into Escherichia coli ET12567/pUZ8002 strain to obtain E. coli ET12567 /pUZ8002+pRAstr
- performing [[the]]a conjugation between the Streptomyces coelicolor A3(2) and the E. coli ET12567/pUZ8002+pRAstr, and
- obtaining a new recombinant cell (SCO+pRAstr).
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