Method for producing allitol
AssigneeAnnikki GmbH
InventorsNicole STAUNIG, Maria DUPONT
Life of this patent
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Abstract
A method for producing allitol by forming in vitro D-psicose from D-fructose present in an aqueous solution by treatment with an epimerase, which D-psicose is reduced in vitro to allitol by treatment with an NAD(P)H-dependent oxidoreductase, while the NAD(P)+ resulting during reduction is enzymatically reduced to NAD(P)H again with a hydrogen donor, characterized in that a secondary alcohol is used as hydrogen donor. (FIG. 1)
Claims15
A method for producing allitol in vitro, the method comprising:
- treating D-fructose present in an aqueous solution with an epimerase to form D-psicose;
- and
- treating D-psicose with an NAD(P)H-dependent oxidoreductase to produce allitol, and enzymatically reducing the resulting NAD(P)+ to NAD(P)H with a hydrogen donor,
- wherein the hydrogen donor is a secondary alcohol.
(canceled)
A method for producing allitol from D-psicose in vitro, the method comprising:
- treating D-psicose present in an aqueous solution with an oxidoreductase to reduce D-psicose to allitol,
- wherein the oxidoreductase comprises an amino acid sequence selected from the group consisting of:
- i) an amino acid sequence having at least 80% identity to SEQ ID NO:
- 2, SEQ ID NO:
- 8, or SEQ ID NO:
- 10,
- ii) an amino acid sequence encoded by a nucleic acid having at least 80% identity to SEQ ID NO:
- 1, SEQ ID NO:
- 7, or SEQ ID NO:
- 9, and
- iii) an amino acid sequence encoded by a nucleic acid that, under stringent conditions, binds to a nucleic acid molecule with the nucleic acid sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 7, or SEQ ID NO:
- 9.
A method for regenerating a co-factor or a functional fragment thereof using a formate dehydrogenase, wherein the formate dehydrogenase comprises an amino acid sequence selected from the group consisting of:
- i) an amino acid sequence having at least 80% identity to SEQ ID NO:
- 12,
- ii) an amino acid sequence encoded by a nucleic acid having at least 80% identity to SEQ ID NO:
- 11, and
- iii) an amino acid sequence encoded by a nucleic acid that, under stringent conditions, binds to a nucleic acid molecule with the nucleic acid sequence of SEQ ID NO:
- 11.
Dependencies are inferred from the claim text.