Transient cellular reprogramming for reversal of cell aging
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsVittorio SEBASTIANO, Tapash J. SARKAR
Life of this patent
Estimating the term…
Abstract
Provided herein are methods and compositions useful in cellular rejuvenation, tissue engineering, and regenerative medicine. Compositions and methods for rejuvenating aged cells and tissues to restore functionality are disclosed. In particular, cells are rejuvenated by transient exposure to non-integrated mRNAs encoding reprograming factors to rejuvenate cells while retaining cells in a differentiated state.
Claims20
-19. (canceled)
Use of a messenger RNA (mRNA) encoding one or more reprogramming factors selected from the group consisting of Oct4, Sox2, Klf4, cMyc, Lin28, and NANOG in the manufacture of a composition for treating a somatic cell by transfecting the somatic cell with the messenger RNA (mRNA) to express the one or more reprogramming factors in the somatic cell to obtain a rejuvenated cell with retention of cellular identity.
Use of an mRNA encoding one or more reprogramming factors selected from the group consisting of Oct4, Sox2, Klf4, cMyc, Lin28, and NANOG in the manufacture of a kit for treating an age-related disease or condition in a subject, the kit comprising a composition for transfecting differentiated cells associated with the age-related disease or condition with the mRNA to express the one or more reprogramming factors in the differentiated cells to obtain rejuvenated cells with retention of cellular differentiation.
Use of a messenger RNA (mRNA) encoding one or more reprogramming factors selected from the group consisting of Oct4, Sox2, Klf4, cMyc, Lin28, and NANOG for transfecting a somatic cell to express the one or more reprogramming factors in the somatic cell to obtain a rejuvenated cell with retention of cellular identity.
Dependencies are inferred from the claim text.