Packaging plasmids for aav production
AssigneeBiogen MA Inc.
InventorsTerrence Michael Dobrowsky, Venkata Soundarya Kandala, Yizhou Zhou
Life of this patent
Estimating the term…
Abstract
The present disclosure provides improved packaging plasmids useful in the production of recombinant adeno-associated viruses.
Claims23
A packaging plasmid comprising a coding region and at least one p5 promoter sequence downstream of a coding region.
The packaging plasmid of any one of the preceding claims, further comprising a pA sequence downstream of the coding region.
The packaging plasmid of any one of the preceding claims, further comprising at least one p5 promoter sequence upstream of the coding region.
The packaging plasmid of any of the preceding claims wherein the p5 promoter sequence is at least 80% identical to any one of SEQ ID NOs:
- 4-15.
The packaging plasmid of any of the preceding claims, wherein the linker sequence is between approximately 1 bp-500 bp in length.
The packaging plasmid of any of the preceding claims, wherein the linker sequence comprises a nucleotide sequence that is at least 80% identical to any one of SEQ ID NOs:
- 16-23.
The packaging plasmid of any of the preceding claims, wherein the coding region comprises a Rep gene.
The packaging plasmid of any of the preceding claims, wherein the coding region comprises a Cap gene.
A method of producing a recombinant adeno-associated viral vector (rAAV) comprising transfecting a host cell with a packaging plasmid of any one of the preceding claims, optionally with a proviral plasmid and/or an adenoviral helper plasmid.
Dependencies are inferred from the claim text.