Method for producing cytoplasmic male sterile line and maintainer line
AssigneeTohoku University
InventorsKinya TORIYAMA, Keisuke IGARASHI, Yuko IWAI, Shinya TAKEDA, Ayumu TAKATSUKA, Tomohiko KAZAMA
Life of this patent
Estimating the term…
Abstract
An object to be achieved by the present invention is to provide a novel system that can produce a cytoplasmic male sterile line and a maintainer line from one and the same species. The present invention provides a method of producing a cytoplasmic male sterile line plant, including a step of knocking out, in a plant having an orf288 gene in mitochondrial genomic DNA thereof and having a pentatricopeptide repeat protein (PPR) gene in nuclear genomic DNA thereof, the PPR gene in the nuclear genomic DNA.
Claims10
A method of producing a cytoplasmic male sterile line plant, comprising a step of knocking out, in a plant having an orf288 gene in mitochondrial genomic DNA thereof and having a pentatricopeptide repeat protein (PPR) gene in nuclear genomic DNA thereof, the PPR gene in the nuclear genomic DNA.
A method of producing a maintainer line plant, comprising the steps of:
- knocking out, in a plant having an orf288 gene in mitochondrial genomic DNA thereof and having a PPR gene in nuclear genomic DNA thereof, the PPR gene in the nuclear genomic DNA;
- and
- knocking out the orf288 gene in the mitochondrial genomic DNA.
A combination of plants of three lines, comprising:
- a restorer line plant, which is a plant having an orf288 gene in mitochondrial genomic DNA thereof and having a pentatricopeptide repeat protein (PPR) gene in nuclear genomic DNA thereof;
- a cytoplasmic male sterile line plant obtained by knocking out, in the restorer line plant, the PPR gene in the nuclear genomic DNA;
- and
- a maintainer line plant obtained by knocking out, in the restorer line plant, the PPR gene in the nuclear genomic DNA and the orf288 gene in the mitochondrial genomic DNA.
Dependencies are inferred from the claim text.