High expression of animal heme protein in plants

A plant expression vector comprising a nucleic acid encoding a recombinant protein, wherein said nucleic acid is operatively linked to a beta-phaseolin (phas) seed-specific promoter, an Arc5 terminator, and a Rb7Mar 3′ Matrix Attachment Region;

• wherein, when the plant expression vector is integrated into a genome plant, induces the production of the recombinant protein in plant seeds in an amount between 3-30% of recombinant protein per total soluble protein in the seed.

A genetically modified plant, plant tissue or plant seed comprising an expression vector for producing recombinant proteins, the expression vector comprising a nucleic acid encoding a recombinant protein, wherein said nucleic acid is operatively linked to a beta-phaseolin (phas) seed-specific promoter, an Arc5 terminator;

• and a Rb7Mar 3′ Matrix Attachment Region;
• wherein the genetically modified plant produces 3-30% of the recombinant proteins per total soluble protein in the plant seeds.

A method to produce 3-30% of recombinant proteins per total soluble protein in plant seeds, the method comprising:

• cultivating a genetically modified plant comprising integrated into its genome an expression vector, an expression vector comprising a nucleic acid encoding a recombinant protein, wherein said nucleic acid is operatively linked to a beta-phaseolin (phas) seed-specific promoter, an Arc5 terminator;
• and a Rb7Mar 3′ Matrix Attachment Region.