Microfluidics-based preparation of cross-linked enzyme aggregates

A method for the preparation of cross-linked enzyme aggregates (CLEAs), the method comprising the steps:

• a) precipitation of at least one enzyme, optionally together with its required co-factor(s), in the presence of a precipitating agent;
• and
• b) reacting the precipitated enzyme(s) with a crosslinking agent to obtain said CLEAs;
• wherein said method is carried out in a microfluidic system, and
• wherein steps a) and b) are not carried out simultaneously.