Compositions Comprising Digestive Enzymes
AssigneeClara Foods Co.
InventorsHalle Redfearn, Harshal Kshirsagar, Kritika Mahadevan, Alexandre Chapeaux, Wesley Rutherford-Jenkins, Joel Andrew Kreps, Isha Joshi, Ying Joy ZHONG, Farnoosh Ayoughi, Ranjan Patnaik, A Samuel Pottinger, Dane Mathias Jacobson, Andrew Keum Min Miyashiro
Life of this patent
Estimating the term…
Abstract
Provided herein are compositions with enhanced protein content, compositions with functional proteins, protein combinations and methods for the preparation thereof.
Claims13
A method for producing a consumable composition via a fermentation process comprising combining a high-activity stable pepsin powdered composition with one or more consumable ingredients comprising steps of:
- (a) providing a microorganism expressing a recombinant pepsinogen, wherein the expressed pepsinogen is secreted by the microorganism into its growth media;
- (b) harvesting the growth media and removing the microorganismal cells therefrom to obtain a liquid starting material;
- (c) lowering the pH of the liquid starting material to less than pH 4.0 to obtain an activated pepsin composition;
- (d) raising the activated pepsin composition to a pH of 5.4 or higher to obtain a high-activity stable pepsin composition;
- and
- (e) isolating the stable pepsin polypeptide from protein and small molecules in the liquid starting material to obtain the high-activity stable pepsin powdered composition having a specific activity from 500 FCC units/mg total protein to 50,000 FCC units/mg total protein;
- (f) combining the high-activity stable pepsin powdered composition with the one or more consumable ingredients to produce a consumable composition;
- and
- wherein step (e) is performed after steps (c) and (d)
- wherein the high-activity stable pepsin powdered composition comprises less than 10% of contaminating proteins.
7-30. (canceled)
A method for producing via fermentation a consumable composition comprising combining a high-activity stable pepsin powdered composition with one or more consumable ingredients comprising steps of:
- (a) providing a microorganism expressing a recombinant pepsinogen, wherein the expressed pepsinogen is secreted by the microorganism into its growth media;
- (b) harvesting the growth media and removing the microorganismal cells therefrom to obtain a liquid starting material;
- (c) lowering the pH of the liquid starting material to less than pH 4.0 to obtain an activated pepsin composition;
- (d) raising the activated pepsin composition to a pH of 4.5 or higher to obtain a high-activity stable pepsin composition;
- and
- (e) isolating the stable pepsin polypeptide from protein and small molecules in the liquid starting material to obtain the high-activity stable pepsin powdered composition having a specific activity from 100 FCC units/mg total protein to 50,000 FCC units/mg total protein;
- (f) combining the high-activity stable pepsin powdered composition with the one or more consumable ingredients to produce a consumable composition;
- and
- wherein step (e) is performed after steps (c) and (d)
- wherein the high-activity stable pepsin powdered composition comprises less than 10% of contaminating proteins.
Dependencies are inferred from the claim text.