Lipase with improved stereoselectivity and lipase-based chiral resolution methods

A protein variant having lipase activity wherein the protein variant is encoded by an amino acid sequence having at least 80% identity with an amino acid sequence shown under SEQ ID No. 1,

• wherein the amino acid sequence of the protein variant differs from the amino acid sequence of SEQ ID NO. 1 in at least one of the following positions:
• i. the amino acid at position 44 is M, W or Y;
• ii. the amino acid at position 51 is N, or M;
• iii. the amino acid at position 52 is L;
• iv. the amino acid at position 53 is S, P, I, E, or A;
• v. the amino acid at position 54 is Q, M, F, G, E, L, T or P;
• vi. the amino acid at position 55 is R, M, D, Y, S, I;
• vii. the amino acid at position 109 is H or F;
• viii. the amino acid at position 110 is T or V;
• ix. the amino acid at position 111 is S;
• x. the amino acid at position 117 is F or S;
• xi. the amino acid at position 121 is V;
• xii. the amino acid at position 122 is Q, A, Y, R, or V;
• xiii. the amino acid at position 153 is Y, D, E, or C;
• xiv. the amino acid at position 160 is E, C, D, P, I, Q, K, M, S, F, A, or N;
• xv. the amino acid at position 179 is C;
• xvi. the amino acid at position 181 is Q;
• xvii. the amino acid at position 184 is G or T;
• xviii. the amino acid at position 211 is E;
• xix. the amino acid at position 212 is S;
• xx. the amino acid at position 216 is K or A;
• xxi. the amino acid at position 234 is K, T or G;
• xxii. the amino acid at position 235 is V or M;
• xxiii. the amino acid at position 236 is T;
• xxiv. the amino acid at position 238 is A, K, D, E, or Q;
• xxv. the amino acid at position 240 is F;
• xxvi. the amino acid at position 289 is S or G;
• xxvii. the amino acid at position 291 is E or W;
• xxviii. the amino acid at position 317 is T;
• xxix. the amino acid at position 320 is E or G;
• xxx. the amino acid at position 321 is F.