Plastic-Degrading Enzymes and Methods of Identifying Same
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsNikita Khlystov, Jennifer R. Cochran
Life of this patent
Estimating the term…
Abstract
Provided are methods of assessing an enzyme for plastic-degrading activity. In certain embodiments, the methods comprise contacting a plastic with the enzyme under conditions suitable for plastic-degrading enzyme activity, and assessing for degradation of the plastic by the enzyme. Also provided are plastic-degrading enzymes and methods of using the same. As a non-limiting example, the plastic-degrading enzymes are polyester-degrading enzymes. As an example, polylactic acid (PLA)-degrading enzymes and methods of using the same. As another example, polyethylene terephthalate (PET)-degrading enzymes and methods of using the same.
Claims33
An enzyme comprising an amino acid sequence having 90% or greater identity to the amino acid sequence set forth in SEQ ID NO:4, wherein the enzyme comprises amino acid substitutions at positions Q1, G3, T29, and E47, according to the numbering of SEQ ID NO:4.
An enzyme comprising an amino acid sequence having 90% or greater identity to the amino acid sequence set forth in SEQ ID NO:3, wherein the enzyme comprises amino acid substitutions at positions A40, Q112 and 1191, according to the numbering of SEQ ID NO:3.
Dependencies are inferred from the claim text.