Photosensitive dna polymerase, use method and use thereof
AssigneeHebei Agricultural University
InventorsQi CHENG, Yang WU, Yukun TIAN, Xizhe SUN, Lifeng HOU, Fanli ZENG
Life of this patent
Estimating the term…
Abstract
A photosensitive DNA polymerase, use method and use thereof, relate to the technical field of enzyme engineering, and address the technical problems of providing a protein and converting same into the photosensitive DNA polymerase. The protein has the amino acid sequence set forth in SEQ ID NO: 3, where X is pAzF, an amino group of the pAzF is linked via a peptide bond to a carboxyl group of lysine at position 4 in SEQ ID NO: 3, and a carboxyl group of the pAzF is linked via a peptide bond to an amino group of histidine at position 5 in SEQ ID NO: 3.
Claims15
A protein, a salt thereof, or a derivative thereof, wherein the protein is any one selected from the group consisting of:
- B1) a protein having the amino acid sequence set forth in SEQ ID NO:
- 3;
- B2) a protein being obtained by substitution, and/or deletion, and/or addition of an amino acid residue in the protein of B1) having not less than 80% identity and retaining the same activity to the protein of B1);
- and
- B3) a fusion protein being obtained by linking a protein tag to an N-terminus and/or a C-terminus of the protein of B1) or the protein of B2);
- wherein
- X is pAzF, an amino group of the pAzF is linked via a peptide bond to a carboxyl group of lysine at position 4 in SEQ ID NO:
- 3, and a carboxyl group of the pAzF is linked via a peptide bond to an amino group of histidine at position 5 in SEQ ID NO:
- 3;
- and
- the pAzF has the structure shown as follows:
A compound, wherein the compound has the structural formula as follows:
- wherein
- V is a polypeptide with an amino acid sequence of MR;
- W is a polypeptide comprising residues 5 to 598 of SEQ ID NO:
- 3;
- Z is an oligonucleotide strand comprising residues 1 to 19 of SEQ ID NO:
- 5;
- wherein
- C represents cytosine;
- A represents adenine;
- a carboxyl group at the carboxyl terminus of the V forms a peptide bond with an amino group of a lysine residue;
- an amino group at the amino terminus of the W forms a peptide bond with a carboxyl group of a pAzF residue;
- and a phosphate group of a 3′-terminal cytosine deoxyribonucleotide of the Z forms a phosphodiester bond with an hydroxyl group on the C atom at position 5 of an adenine deoxyribonucleotide.
Dependencies are inferred from the claim text.