New fucosyltransferase for in vivo synthesis of complex fucosylated human milk oligosaccharides
OwnerManos PAPADAKISinventor
Life of this patent
Estimating the term…
Abstract
The present invention relates to the production of fucosylated Human Milk Oligosaccharides (HMOs), and in particular to the production of complex fucosylated HMOs with five or more monosaccharide units such as LNFP-II, LNFP-III and LNDFH-I from precursor oligosaccharides and the genetic engineering of suitable cells for use in said production, as well as to methods for producing said fucosylated HMOs.
Claims21
A genetically engineered cell capable of producing one or more fucosylated human milk oligosaccharides (HMOs), comprising a recombinant nucleic acid sequence encoding a fucosyltransferase with α-1,3(4)-fucosyltransferase activity, wherein the fucosyltransferase is Med1 with an amino acid sequence according to SEQ ID NO:
- 1, or a functional homologue thereof with an amino acid sequence that is at least 80% identical to SEQ ID NO:
- 1.
The genetically engineered cell according to any of the preceding claims, wherein the cell, in fermentative conditions, produces a mixture of HMOs consisting essentially of
- a. LNFP-II, pLNH2 and LNT,
- b. LNFP-II, LNT and 3FL,
- c. LNFP-II, LNT, 3FL and pLNH2,
- d. LNT-II, LNFP-II, LNT, 3FL and pLNH2,
- e. LNFP-III and LNnT,
- f. LNFP-III, LNnT and pLNnH,
- g. LNFP-III and 3FL, or
- h. LNDFH-I, LNFP-I and 2′FL.
-15. (canceled)
A mixture of HMOs consisting essentially of
- a. LNFP-II, pLNH2 and LNT,
- b. LNFP-II, LNT, 3FL and pLNH2,
- c. LNT-II, LNFP-II, LNT, 3FL and pLNH2,
- d. LNFP-III and LNnT,
- e. LNFP-III, LNnT and pLNnH,
- f. LNFP-III and 3FL, or
- g. LNDFH-I, LNFP-I and 2′FL.
(canceled)
Dependencies are inferred from the claim text.