Methods and Compositions for the Enrichment of Nucleic Acid Containing AAV Capsids
AssigneeBrammer Bio, LLC
InventorHetal Brahmbhatt
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Abstract
The present disclosure exemplifies methods and compositions by which full recombinant adeno-associated virus (AAV) capsids are enriched from a composition having both empty and full AAV capsids. Demonstrated herein is that full AAV capsids can be enriched using an isocratic step gradient wherein the elution buffers are pre-made and do not, therefore, require on-line formulation. This advancement improves the ease of automation at manufacturing scale by reducing operational errors or failures and in turn lost AAV therapeutic batches.
Claims24
A method for enriching for full AAV capsids, the method comprising loading a sample suspected of comprising empty and full AAV capsids onto an anion exchange chromatography medium, differentially eluting the full AAV capsids and the empty AAV capsids with an elution buffer, the composition of the elution buffer is not prepared in-line.
Dependencies are inferred from the claim text.