Metabolic selection for glycogen-storing cells in vitro
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsLay Teng Ang, Kevin J. Liu, Kyle M. Loh, Nicole Min Qian Pek, Irving L. Weissman
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Abstract
Methods are provided for in vitro selection of metabolic selection of metabolically selectable mammalian cells that don't require exogenous glucose and glutamine in culture. Such cells may be glycogen-storing mammalian cells, including without limitation hepatocytes. Metabolic selection provides an approach to rapidly kill non-selectable cells by withholding specific nutrients from the culture medium.
Claims16
A method to select for metabolically selectable mammalian cells that don't require exogenous glucose and glutamine in culture, the method comprising:
- culturing a mixed population of cells comprising metabolically selectable mammalian cells and non-metabolically selectable cells in medium substantially free of glucose and glutamine for a period of time sufficient to kill non-metabolically selectable mammalian cells in the culture.
A culture medium substantially free of glucose and glutamine, comprising a mixed population of cells comprising metabolically selectable mammalian cells and non-metabolically selectable mammalian cells.
Dependencies are inferred from the claim text.