Induction/differentiation medium for producing of mesenchymal stem cells and application thereof
AssigneeHunan Meibo Biomedical Co., Ltd
InventorsE XIAO, Qiushi LIU
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Abstract
The present disclosure belongs to the field of stem cell biology, relates to lineage-specific differentiation of human pluripotent or embryonic stem cells, and specifically relates to a induction/differentiation medium for producing of mesenchymal stem cells and application thereof. The induction/differentiation medium is used for directly inducing differentiation of human induced pluripotent stem cells or embryonic stem cells into mesenchymal stem cells. The induction/differentiation medium includes a mesenchymal stem cell basal medium and additives. The additives include: 2 to 10 μM of a TGF-β inhibitor, 2 to 10 μM of a GSK3β inhibitor, and 5 to 10 μM of a ROCK inhibitor.
Claims16
A induction/differentiation medium for producing of mesenchymal stem cells, wherein the induction/differentiation medium is used for directly inducing differentiation of human induced pluripotent stem cells or embryonic stem cells into mesenchymal stem cells, and the induction/differentiation medium comprises:
- a mesenchymal stem cell basal medium and additives, wherein the additives include:
- 2 to 10 μM of transforming growth factor-β (TGF-β) inhibitor, 2 to 10 μM of glycogen synthase kinase-3β (GSK3β) inhibitor, and 5 to 10 μM of rho-associated coiled-coil containing protein kinase (ROCK) inhibitor.
Dependencies are inferred from the claim text.