Safe and efficient method for hypoxia-induced adipogenic differentiation of cells and use of method

Abstract

The present invention discloses a safe and efficient method for hypoxia-induced adipogenic differentiation of cells and use thereof, belonging to the technical field of cell culture. The method involves expanding cells to a certain number in a reactor in a suspension environment, and inducing adipogenic differentiation of the cells in suspension by reducing a dissolved oxygen level to hypoxia. The method induces adipogenic differentiation of cells solely through physical factors. Compared to traditional chemical induction adipogenic differentiation methods, no exogenous reagents are introduced during a differentiation process, and food safety is ensured. By using the method, cell adipogenic differentiation can be completed in 4-6 days, and expansion and adipogenic differentiation of cells can be completed in about 10 days, thus accelerating differentiation. Moreover, adipogenic differentiation can be completed in a large-scale reactor of, for example, 200 L, and the method can be applied to large-scale preparation of cell-cultured meat.

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