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US 2026/0258357 A1Published application
Method for the cryopreservation of t regulatory cells
OwnerEverett Hurteau Meyer, Anna Pavlovainventors
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Abstract
Compositions and methods are provided that relate to the cryopreservation of human Treg cells. The Treg cells can be provided for therapeutic use, e.g. administration to a patient in need thereof. The cryopreservation methods disclosed herein provide for improved Treg viability, Treg counts, and Treg function. In particular, the methods feature short periods of time in culture before and after freezing in order to minimize in vitro changes in the phenotype and function of the cells.
Claims18
A method of cryopreserving regulatory T cells (Tregs) for enhanced viability, cell recover and function, the method comprising:
- performing in a period of less than about 72 hours pre-freezing steps of:
- (a) collecting cells from a human donor;
- (b) selecting for Tregs;
- (c) activating Tregs in vitro;
- (d) cryopreserving Tregs;
- and
- performing in a period of less than about 72 hours post-thaw steps of:
- (e) thawing Tregs,
- (f) activating Tregs in vitro.
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