Induced photoreceptor cells and methods for their production
AssigneeRheinische Friedrich-Wilhelms-Universität Bonn
InventorsVolker Busskamp, Marta Zuzic, Anka Kempe
Life of this patent
Estimating the term…
Abstract
A method for producing induced photoreceptor cells from an initial cell is provided, the method includes providing one or more transcription factors (TFs) including at least GON4L to the initial cell. In some versions, the initial cell is a human induced pluripotent stem cell (iPSC). In other embodiments the method includes providing the TFs OTX2 and/or NEUROD1 to the initial cell. Cells produced and obtainable by the method are also provided, the use of these cells as a medicament in the treatment of retinopathy, vectors for inducing the photoreceptor cells and combinations of transcription factors intended for this use.
Claims11
A method for producing an induced photoreceptor cell from an initial cell, the method comprising:
- providing transcription factors (TFs) comprising GON4L, OTX2, and NEUROD1 to the initial cell, wherein the initial cell is one of a pluripotent or multipotent mammalian cell, a fibroblast, a retinal progenitor cell (RPC), a retinal pigment epithelium (RPE) cell, a Mueller Glia cell or other cell found in the eye or retina with the proviso that the initial cell is not a photoreceptor cell;
- and
- allowing sufficient time to induce a reprogramming of the initial cell to the induced photoreceptor cell.
An expression vector system comprising one or more nucleic acid sequences operably coupled to one or more promoters, wherein said sequences encode one or more transcription factors (TFs) comprising at least GON4L, OTX2, NEUROD1, and optionally miR-182 and/or miR-183.
Dependencies are inferred from the claim text.