Microenvironment-simulated cell culture system and microenvironment-simulated cell culturing method

A microenvironment-simulated cell culture system, comprising:

• a cell culture chip, comprising:
• at least one cell culture chamber, wherein the at least one cell culture chamber comprises a first side portion and a second side portion, and the first side portion and the second side portion are respectively disposed on two ends of the at least one cell culture chamber along a long axis of the cell culture chip;
• at least one fluid storing well connected to the at least one cell culture chamber;
• at least three membrane pump units connected to the at least one fluid storing well, wherein the at least three membrane pump units are arranged in sequence;
• at least one air transporting channel connected among the at least three membrane pump units, wherein the at least one air transporting channel is substantially s-shaped, and a width of the air transporting channel is 0.06 mm to 0.12 mm;
• at least one valve connected to the air transporting channel;
• and
• at least two air transporting pores, wherein one of the at least two air transporting pores is connected to the at least three membrane pump units, and the other one of the at least two air transporting pores is connected to the at least one valve;
• a fluid storage device connected to the at least one fluid storing well, wherein the fluid storage device is for supplying a cell culture medium to the at least one fluid storing well;
• and
• an air pump connected to the at least two air transporting pores, wherein the at least one valve is connected between the air transporting channel and the air pump;
• wherein the at least one cell culture chamber is substantially a long-stripped slot, two long sides of the at least one cell culture chamber are parallel to the long axis of the cell culture chip, a length ratio of a short side of the at least one cell culture chamber to one of the long sides of the at least one cell culture chamber is 1:1 to 1:4, and a length of the one of the long sides of the at least one cell culture chamber is 1.3 cm to 2.0 cm.