Chimeric antigen receptors for direct and indirect targeting of fibronectin-positive tumors
AssigneeSt. Jude Children's Research Hospital
InventorsStephen Gottschalk, Jessica Wagner, Timothy Isham Shaw, Jinghui Zhang
Life of this patent
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Abstract
The application relates to a chimeric antigen receptor that directly and/or indirectly targets cells and their uses in tumor immunotherapy. The application also relates to polynucleotides that encode the chimeric antigen receptor and optionally accessory genes, vectors, and host cells comprising the chimeric antigen receptor and optionally a second antigen targeting moiety (e.g., a second chimeric antigen receptor or a bispecific molecule). The application also relates to methods for preparing host cells comprising the chimeric antigen receptor and optionally the second antigen targeting moiety.
Claims27
-51. (canceled)
A chimeric antigen receptor (CAR) comprising,
- (a) an extracellular target-binding domain comprising a Fibronectin Extradomain B (FN-EDB)-binding moiety,
- (b) a hinge domain,
- (c) a transmembrane domain, and
- (d) a cytoplasmic domain comprising
- (i) optionally one or more costimulatory domains, and
- (ii) a lymphocyte activation domain.
An isolated host cell, comprising a polynucleotide sequence encoding a chimeric antigen receptor (CAR) targeting a Fibronectin Extradomain B (FN-EDB) and at least one additional polynucleotide sequence encoding a second gene, wherein the CAR comprises:
- (a) an extracellular target-binding domain comprising a Fibronectin Extradomain B (FN-EDB)-binding moiety,
- (b) a hinge domain,
- (c) a transmembrane domain, and
- (d) a cytoplasmic domain comprising
- (i) optionally one or more costimulatory domains, and
- (ii) a lymphocyte activation domain.
An isolated host cell, comprising a chimeric antigen receptor (CAR) comprising the amino acid sequence of SEQ ID NO:
- 95, or an amino acid sequence having at least 80% identity thereof.
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-70. (canceled)
-76. (canceled)
-86. (canceled)
Dependencies are inferred from the claim text.