Anti-toh1 antibodies and methods of use thereof
AssigneeThe Cleveland Clinic Foundation
InventorJeannette Messer
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Abstract
The present disclosure relates to anti-ToH1 antibodies, methods of generating anti-ToH1 antibodies, and methods of use of anti-ToH1 antibodies for diagnosis and treatment of microbial disease and/or chronic inflammatory disease, including microbial infection and microbe-associated chronic inflammatory conditions.
Claims36
An antibody that binds to target of HGMB1 (ToH1), the antibody comprising:
- a) a heavy chain variable domain comprising complementary determining regions HCDR1, HCDR2, and HCDR3 and
- b) and light chain variable domain comprising complementary determining regions LCDR1, LCDR2, and LCDR3, wherein
- i. HCDR1, HCDR2, and HCDR3 comprise amino acid sequences having at least 80% sequence identity to SEQ ID NO:
- 11, SEQ ID NO:
- 12, and SEQ ID NO:
- 13, respectively, and LCDR1, LCDR2, and LCDR3 comprise amino acid sequences having at least 80% sequence identity to SEQ ID NO:
- 14, SEQ ID NO:
- 15, and SEQ ID NO:
- 16, respectively;
- ii. HCDR1, HCDR2, and HCDR3 comprise amino acid sequences having at least 80% sequence identity to SEQ ID NO:
- 17, SEQ ID NO:
- 18, and SEQ ID NO:
- 19, respectively, and LCDR1, LCDR2, and LCDR3 comprise amino acid sequences having at least 80% sequence identity to SEQ ID NO:
- 20, SEQ ID NO:
- 21, and SEQ ID NO:
- 22, respectively;
- or
- iii. HCDR1, HCDR2, and HCDR3 comprise amino acid sequences having at least 80% sequence identity to SEQ ID NO:
- 23, SEQ ID NO:
- 24, and SEQ ID NO:
- 25, respectively, and LCDR1, LCDR2, and LCDR3 comprise amino acid sequences having at least 80% sequence identity to SEQ ID NO:
- 26, SEQ ID NO:
- 27, and SEQ ID NO:
- 28, respectively.
An antibody that binds to target of HGMB1 (ToH1), wherein the antibody is a nanobody having at least 80% identity to SEQ ID NO:
- 29 or SEQ ID NO:
- 30.
A method of treating or preventing microbial disease and/or chronic inflammatory disease in a subject, the method comprising providing to the subject an antibody that binds to target of HGMB1 (ToH1).
A method of diagnosing microbial disease and/or chronic inflammatory disease in a subject, the method comprising determining levels of target of HGMB1 (ToH1) in a sample obtained from a subject, and determining that the subject has a microbial disease when the level of ToH1 in the sample are equal to or above a threshold value.
A method of generating an antibody that binds to target of HGMB1 (ToH1), the method comprising:
- a) sequentially immunizing a host with two or more unique peptides, each of the two or more unique peptides having the motif [S/T]xExPx[I/V], wherein each x is a variable amino acid, and
- b) isolating antibodies generated in response to immunization.
Dependencies are inferred from the claim text.