Hplc free purification of peptides by the use of new capping and capture reagents
AssigneeRoche Diagnostics Operations, Inc.
InventorsFrank Bergmann, Sebastian Johannes Pomplun
Life of this patent
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Abstract
The present disclosure relates to the use of a capping and capture reagent in solid phase peptide synthesis. The present disclosure further relates to a method of solid phase peptide synthesis, wherein a capping and capture reagent according to the present disclosure is used. The present disclosure further relates to a method for purification of a (full-length) synthetic peptide via use of a capping and capture reagent according to the present disclosure. The present disclosure also relates to a kit comprising a capping and capture reagent according to the present disclosure and an amino oxy resin or a hydrazine resin and the use of the kit.
Claims8
1-15. (canceled)
A composition comprising:
- a capping and capture (cap-capt) reagent for a solid phase peptide synthesis, said capping and capture reagent comprising a capping moiety and a capture moiety, and
- an incomplete failure peptide sequence,
- wherein
- individual amino acids are coupled to each other via Fmoc-chemistry in the solid phase peptide synthesis, and
- said capping moiety is a carboxylic acid or an activated carboxylic acid and said capture moiety is a protected carbonyl group.
Dependencies are inferred from the claim text.