Colored coverlay dielectrics in digital microfluidics
AssigneeNicoya Lifesciences Inc.
InventorsHarish KRISHNAKUMAR, Arjun SUDARSAN
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Abstract
Provided herein are devices, systems and methods comprising a digital microfluidics (DMF) cartridge, the DMF cartridge comprising: a top plate, wherein the top plate comprises a top plate substrate; a bottom plate, wherein the bottom plate comprises a colored coverlay substrate; and a droplet operation gap between the top plate and the bottom plate.
Claims22
A digital microfluidics (DMF) cartridge, the DMF cartridge comprising:
- a top plate, wherein the top plate comprises a top plate substrate;
- a bottom plate, wherein the bottom plate comprises a bottom plate substrate and a colored coverlay substrate;
- and
- a droplet operation gap between the top plate and the bottom plate.
A method for analyzing an analyte of interest using an optical detection operation, the method comprising:
- (a) providing a digital microfluidic (DMF) cartridge, where the DMF cartridge comprises:
- (i) a top plate, wherein the top plate comprises a top plate substrate;
- (ii) a bottom plate, wherein the bottom plate comprises a colored coverlay substrate having a plurality of electrodes operable to perform droplet operations;
- and
- (iii) a droplet operation gap between the top plate and the bottom plate;
- (b) providing a sample droplet to be analyzed for the presence of the analyte of interest;
- (c) performing droplet operations on the droplet sample, wherein the performing comprises moving the droplet sample to a sensor well in the DMF cartridge;
- (d) performing an optical detection operation by illuminating the droplet sample in the sensor well using an illumination source;
- (e) detecting light emitting from the droplet sample using an optical measurement device;
- and
- (f) using a processor, detecting the presence of an analyte and/or determining the concentration of the analyte by analyzing the detected light.
-37. (canceled)
A method for detecting an analyte using colorimetric-based optical detection operations, the method comprising:
- (a) providing a digital microfluidic (DMF) cartridge, where the DMF cartridge comprises:
- (i) a top plate, wherein the top plate comprises a top plate substrate;
- (ii) a bottom plate, wherein the bottom plate comprises a colored coverlay substrate having a plurality of electrodes operable to perform droplet operations, wherein the color of the colored coverlay substrate is white, light gray, silver or light yellow;
- and
- (iii) a droplet operation gap between the top plate and the bottom plate;
- (b) providing a sample droplet to be analyzed for the presence of an analyte of interest;
- (c) performing droplet operations on the sample droplet to move the sample droplet to a sensor well in the DMF cartridge;
- (d) performing a colorimetric-based optical detection operation by illuminating the sample droplet using a colorimetric-based illumination source;
- (e) detecting light emitted from the sample droplet at one or more color specific wavelengths using an optical measurement device;
- and
- (f) using a processor, detecting the presence of an analyte and/or determining the concentration of the analyte by analyzing the detected light.
-58. (canceled)
Dependencies are inferred from the claim text.