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US 2026/0256899 A1Published application
Porphyromonas gingivalis antigenic constructs
AssigneeSanofi
InventorsYves GIRERD-CHAMBAZ, Andreas KARLSSON, Fabienne PIRAS-DOUCE, Khang ANH TRAN
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Abstract
This invention relates to compositions (e.g. vaccine compositions) which can be used to immunise against P. gingivalis infections. The compositions comprise P. gingivalis antigens and antigen combinations which can be used to immunise against P. gingivalis, used in the form of nucleic acids (e.g. mRNAs) encoding antigenic proteins or in the form of recombinant protein antigens.
Claims25
A nucleic acid comprising a nucleotide sequence encoding a polypeptide, wherein the polypeptide comprises:
- i) at least a portion of a Porphyromonas gingivalis Lys-specific proteinase (Kgp) catalytic domain;
- ii) at least a portion of a Porphyromonas gingivalis Kgp domain of unknown function 2436 (DUF2436);
- iii) at least a portion of a Porphyromonas gingivalis Kgp K1 adhesin domain;
- iv) a first Porphyromonas gingivalis Kgp portion that comprises an adhesin binding motif 1 (ABM1) and a first Porphyromonas gingivalis Kgp portion that comprises an adhesin binding motif 2 (ABM2);
- v) a second Porphyromonas gingivalis Kgp portion that comprises an ABM1 and a second Porphyromonas gingivalis Kgp portion that comprises an ABM2;
- and
- vi) a Porphyromonas gingivalis Kgp portion that comprises an adhesin binding motif 3 (ABM3).
4 more elements
5-7. (canceled)
(canceled)
A nucleic acid comprising a nucleotide sequence encoding a polypeptide, wherein the polypeptide comprises:
- i) at least a portion of a Porphyromonas gingivalis Arg-specific proteinase A (RgpA) or Arg-specific proteinase B (RgpB) catalytic domain, for example at least a portion of the Porphyromonas gingivalis Arg-specific proteinase A (RgpA) catalytic domain;
- ii) at least a portion of a Porphyromonas gingivalis RgpA DUF2436;
- iii) at least a portion of a Porphyromonas gingivalis RgpA K1 adhesin domain;
- iv) a first Porphyromonas gingivalis RgpA portion that comprises an ABM1, and a first Porphyromonas gingivalis RgpA portion that comprises an ABM2;
- v) a second Porphyromonas gingivalis RgpA portion that comprises an ABM1, and a second Porphyromonas gingivalis RgpA portion that comprises an ABM2;
- and
- vi) a Porphyromonas gingivalis RgpA portion that comprises an ABM3.
4 more elements
15-17. (canceled)
19-21. (canceled)
A composition comprising:
- (a) a first nucleic acid encoding a polypeptide comprising:
- i) at least a portion of a Kgp catalytic domain, optionally wherein the at least a portion of a Kgp catalytic domain comprises a full-length Kgp catalytic domain;
- ii) at least a portion of a Kgp DUF2436;
- iii) at least a portion of a Kgp K1 adhesin domain, optionally wherein the at least a portion of a Kgp K1 adhesin comprises a full-length Kgp K1 adhesin domain;
- iv) a first Kgp portion that comprises an ABM1 and a first Kgp portion that comprises an ABM2;
- v) a second Kgp portion that comprises an ABM1 and a second Kgp portion that comprises an ABM2;
- and
- vi) a Kgp portion that comprises ABM3;
- wherein the domains are positioned from the N-terminus to the C-terminus of the polypeptide in the following order:
- Kgp catalytic domain;
- first Kgp portion comprising ABM1;
- Kgp DUF2436;
- first Kgp portion comprising ABM2;
- second Kgp portion comprising ABM1;
- Kgp portion comprising ABM3;
- Kgp K1;
- second Kgp portion comprising ABM2;
- optionally wherein the glycosylation site corresponding to the glycosylation site N691-T693 in the WT Kgp sequence (SEQ ID NO:
- 157) is mutated by a T693A substitution;
- and wherein the glycosylation site corresponding to the glycosylation site N950-T952 in the WT Kgp sequence (SEQ ID NO:
- 157) is mutated by a T952A substitution;
- and
- (b) a second nucleic acid encoding a polypeptide comprising:
- i) at least a portion of a RgpA catalytic domain;
- ii) at least a portion of a RgpA DUF2436;
- iii) at least a portion of a RgpA K1 adhesin domain;
- iv) a first RgpA portion that comprises an ABM1 and a first RgpA portion that comprises an ABM2;
- v) a second RgpA portion that comprises an ABM1 and a second RgpA portion that comprises an ABM2;
- vi) a RgpA portion that comprises ABM3;
- vii) at least a portion of a RgpA K2 adhesin domain;
- wherein the domains are positioned from the N-terminus to the C-terminus of the polypeptide in the following order:
- RgpA catalytic domain;
- first RgpA portion comprising ABM1;
- RgpA DUF2436;
- first RgpA portion comprising ABM2;
- second RgpA portion comprising ABM1;
- RgpA portion comprising ABM3;
- RgpA K1;
- RgpA K2;
- second RgpA portion comprising ABM2.
37 more elements
A composition comprising:
- (a) a first polypeptide comprising:
- i) at least a portion of a Kgp catalytic domain, optionally wherein the at least a portion of a Kgp catalytic domain comprises a full-length Kgp catalytic domain;
- ii) at least a portion of a Kgp DUF2436;
- iii) at least a portion of a Kgp K1 adhesin domain, optionally wherein the at least a portion of a Kgp K1 adhesin comprises a full-length Kgp K1 adhesin domain;
- iv) a first Kgp portion that comprises an ABM1 and a first Kgp portion that comprises an ABM2;
- v) aa second Kgp portion that comprises an ABM1 and a second Kgp portion that comprises an ABM2;
- and
- vi) a Kgp portion that comprises ABM3;
- wherein the domains are positioned from the N-terminus to the C-terminus of the polypeptide in the following order:
- Kgp catalytic domain;
- first Kgp portion comprising ABM1;
- Kgp DUF2436;
- first Kgp portion comprising ABM2;
- second Kgp portion comprising ABM1;
- Kgp portion comprising ABM3;
- Kgp K1;
- second Kgp portion comprising ABM2;
- optionally wherein the glycosylation site corresponding to the glycosylation site N691-T693 in the WT Kgp sequence (SEQ ID NO:
- 157) is mutated by a T693A substitution;
- and wherein the glycosylation site corresponding to the glycosylation site N950-T952 in the WT Kgp sequence (SEQ ID NO:
- 157) is mutated by a T952A substitution;
- and
- (b) a second polypeptide comprising:
- i) at least a portion of a RgpA catalytic domain;
- ii) at least a portion of a RgpA DUF2436;
- iii) at least a portion of a RgpA K1 adhesin domain;
- iv) a first RgpA portion that comprises an ABM1 and a first RgpA portion that comprises an ABM2;
- v) a second RgpA portion that comprises an ABM1 and a second RgpA portion that comprises an ABM2;
- vi) a RgpA portion that comprises ABM3;
- vii) at least a portion of a RgpA K2 adhesin domain;
- wherein the domains are positioned from the N-terminus to the C-terminus of the polypeptide in the following order:
- RgpA catalytic domain;
- first RgpA portion comprising ABM1;
- RgpA DUF2436;
- first RgpA portion comprising ABM2;
- second RgpA portion comprising ABM1;
- RgpA portion comprising ABM3;
- RgpA K1;
- RgpA K2;
- second RgpA portion comprising ABM2.
37 more elements
Dependencies are inferred from the claim text.