Antimicrobial recombinant live products and methods
AssigneeGeneral Probiotics, Inc.
InventorsYiannis N. Kaznessis, Kathryn Gayle Kruziki, Samuel Weber Morris
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Abstract
This disclosure relates to antimicrobial recombinant live therapeutics that are Bacillus species plural (spp.) that colonize the gastrointestinal tract of animals. The Bacillus live therapeutics are genetically modified to produce antimicrobial peptides such as class II bacteriocins. The Bacillus live therapeutics form spores and are fed to animals. The Bacillus live therapeutics lower carriage of Clostridia perfringens (C. perfringens) in fed animals and are used to prevent and control disease conditions caused by C. perfringens, for example, necrotic enteritis.
Claims28
A composition for treating a Clostridia perfringens-induced disease condition comprising a Bacillus bacterium isolated from an intestinal tract of an animal and transformed with an exogenous polynucleotide, wherein the exogenous polynucleotide comprises a heterologous promoter operably linked to a polynucleotide that encodes a secretion tag fused to an antimicrobial peptide with bacteriolytic or bacteriostatic activity against Clostridia perfringens.
The composition of any preceding claim, wherein the Bacillus bacterium does not contain the genes of proteases NprE, NprB, AprE, WprA, Vpr, Bpr, Epr, HtrA, and HtrB with greater than 70%, greater than 60%, or greater than 50% homology to these genes present in Bacillus subtilis 168.
A composition of any preceding claim, wherein the antimicrobial peptide is a Class II bacteriocin.
The composition of any preceding claim, wherein the Clostridia perfringens-induced disease is necrotic enteritis.
The composition of any preceding claim, wherein the heterologous promoter is the constitutive promoter p43.
The composition of any preceding claim, wherein the exogenous polynucleotide that encodes the antimicrobial protein is a plasmid.
The composition of any preceding claim further comprising a pharmaceutically acceptable carrier.
Dependencies are inferred from the claim text.